EFFECT OF6-HYDROXYFLAVONE ONCISPLATININDUCED HISTOPATHOLOGICAL AND BIOCHEMICAL CHANGES IN LIVER OF SPRAGUE-DAWELY RATS
DOI:
https://doi.org/10.33279/jkcd.v9i04.405Keywords:
Chemotherapy and hepatotoxicity, Flavonoids, Hepatoprotective natural products, Cisplatin and oxidative stress, Antioxidants, Cisplatin-induced hepatotoxicity.Abstract
Objective: Chemotherapeutic agents like cisplatin benefits cancer patients by decreasing metastasis, relapse and increase the overall survivability.The clinical effectiveness of these beneficial chemotherapeutics is greatly limited by occurrence of side-effects including hepatotoxicity. Flavonoids possess potent hepatoprotective effects and exhibit antioxidant property. In this study, the hepatoprotective effect of the flavonoid, 6-hydroxyflavone was investigated against cisplatin induced liver damage in rats.
Materials & Methods: 6-Hydroxyflavone (25 and 50 mg/kg) and silymarin (100 mg/kg)were administered for 14 days and hepatotoxicity was induced by a single injection of cisplatin (7.5 mg/kg) on the 10th day of experiment. The liver tissues were examined for histopathologicalandbiochemical changes.
Results: Cisplatin produced histopathological changes of sinusoidal dilatation and steatosis and elevated the serumalanine aminotransferase, aspartate aminotransferase and alkaline phosphatase levels. Cisplatin generated oxidative stress in the liver which was observed from significant increase lipid peroxidation and reduction in the level of antioxidant mechanisms of glutathione, catalase and superoxide dismutase. Administration of6-hydroxyflavone and silymarin protected the liver from cisplatin-induced degenerative changes and reduced the escalated levels of biochemical parameters. These protective doses also significantly inhibited the generation of oxidative stress as significant attenuation of lipid peroxidation and an increase in the antioxidant status were observed in the cisplatin-injected animals.
Conclusion: These findings suggest that 6-hydroxyflavonehas prospective hepatoprotective effect mediated by inhibition of oxidative stress in the hepatocytes and thus can be considered when treating cancer patients with chemotherapeutic drugs including cisplatin.
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Copyright (c) 2019 Zia Ud Din, Shazia Iftikhar, Zainab Rehman, Muhammad Harris, Sadaf Ambreen, Munila Shabnum Khattak, Muhammad Shahid, Rahimullah, Sobia Haris
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