HISTOMORPHOLOGICAL EFFECTS OF SALICYLIDENE SALICYL HYDRAZIDE ON LIVER IN SPRAGUE DAWLEY RATS

Authors

  • Waqar Ahmad Department of Anatomy, Khyber Medical College Peshawar, KP Pakistan
  • Falak Naz Department of Anatomy, Khyber Medical College Peshawar, KP Pakistan
  • Bareerah Khan Department of Anatomy, Khyber College of Dentistry, Peshawar, KP Pakistan
  • Asif Kamal Department of Anatomy, Khyber Medical College Peshawar, KP Pakistan
  • Shazia Iftikhar Department of Anatomy, Khyber Medical College Peshawar, KP Pakistan
  • Zainab Rahman Department of Anatomy, Khyber Medical College Peshawar, KP Pakistan

DOI:

https://doi.org/10.33279/jkcd.v10i04.196

Keywords:

salicylidene salicyl hydrazide (SCS), liver, toxicity

Abstract

Objective:

This study was aim to identify histomorphological changes in the liver of Sprague dawley rats after exposure to Saliyclidene salicylhydrazide and dose dependent histomorphological changes in the liver.

Materials and Methods:

This study was started from Mar 2019, to June 2019. In this study, acute toxicity of seven days, the sixteen Sprague dawley female rats were selected. They were divided into four groups. Two test groups (A & B). Group A was given 25mg/kg SCS, while in group B 50mg/kg of SCS was given. Positive control group (C) was given paracetamol 300mg/kg. Group D was a negative control group. Each group had four animals. After seven days of exposure to SCS through oral route all the animals were euthanized. Their livers were harvested and processed for preparation of histological slides. These slides were then analyzed by microscopy and histomorphological changes were recorded and analyzed according to Roenigk classification system.

Results:

Necroinflammatory changes were observed in Group A, B and C compared to Group D(-control). However, difference between Group A and B was not significant. Nuclear pleomorphism was observed in hepatocytes of Group A, B and C compared to group D(control). Pleomorphic changes in the intervention group A & B were same. Steatosis was observed in group A, B and C compared to Group D. Steatosis in group A and B were same while in group C it was relatively more significant. The overall grading according to ROENIGK classification system showed significantly higher toxicity in group B, compared to other intervention groups (A, B, C) and control group D.

Conclusion:

Significant effects of SCS were observed in liver of Sprague dawley rats, however, the toxic effects were less severe. Liver Parenchyma and architecture were preserved.

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Published

2020-12-31

How to Cite

Waqar Ahmad, Falak Naz, Bareerah Khan, Asif Kamal, Shazia Iftikhar, & Zainab Rahman. (2020). HISTOMORPHOLOGICAL EFFECTS OF SALICYLIDENE SALICYL HYDRAZIDE ON LIVER IN SPRAGUE DAWLEY RATS. JOURNAL OF KHYBER COLLEGE OF DENTISTRY, 10(04), 72–76. https://doi.org/10.33279/jkcd.v10i04.196

Issue

Vol. 10 No. 04 (2020): Journal of Khyber College of Dentistry

Section

Original Article

License

Copyright (c) 2020 Waqar Ahmad, Falak Naz, Bareerah Khan, Asif Kamal, Shazia Iftikhar, Zainab Rahman

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