TO INVESTIGATE THE IN-VITRO EFFECT OF ERYTHROPOIETIN ON CELL LINES USING MTT ASSAY

Authors

  • Sajid Hanif Baqai Dental College, Karachi
  • Zahur Qayyum Women Medical College, Abbottabad
  • Muhammad Nauman Sheikh Baqai Dental College, Karachi
  • Shahid Khan Women Medical College, Abbottabad

DOI:

https://doi.org/10.33279/jkcd.v4i2.263

Keywords:

Erythropoietin, MTT assay, squamous cell carcinoma.

Abstract

Objective: The objective of this study was to investigate the effect of Erythropoietin on cellular proliferation in SCC-25, TR146 and FIBS cells lines.

Methodology: This study focuses on the effects of Erythropoietin on 3 cell lines(SCC-25, TR146 and FIBS) when applied for 24 hours respectively. MTT assay was carried out using a range of Erythropoietin concentrations (1, 10, 25 units). Serum free medium was used as a control.

Results: In this study Erythropoietin significantly increased the cell proliferation of SCC-25 and FIBS with 1 and 10unit/ml but had no effect on TR146 cells, while 25 unit Erythropoietin showed very little effect in increase cell viability in both SCC-25 and FIBS.

Conclusions: This study has confirmed that all the concentration of Erythropoietin used had an effect on SCC-25 and FIBS cell viability but erythropoietin had no effect on TR146 cell viability. 

 

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Published

2014-06-30

How to Cite

Sajid Hanif, Zahur Qayyum, Muhammad Nauman Sheikh, & Shahid Khan. (2014). TO INVESTIGATE THE IN-VITRO EFFECT OF ERYTHROPOIETIN ON CELL LINES USING MTT ASSAY. JOURNAL OF KHYBER COLLEGE OF DENTISTRY, 4(2), 24–28. https://doi.org/10.33279/jkcd.v4i2.263

Issue

Vol. 4 No. 2 (2014): Journal of Khyber College of Dentistry

Section

Original Article

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Copyright (c) 2014 Sajid Hanif, Zahur Qayyum, Muhammad Nauman Sheikh, Shahid Khan

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