MORPHOLOGICAL EFFECT OF NICOTINE ON RECONSTITUTED MODEL OF AN INFLAMED ORAL MUCOSA AN IN VITRO STUDY

Authors

  • Muhammad Nauman Sheikh Department of Oral Pathology, College of Dentistry Baqai Medical University, Karachi.
  • Asmat Ullah Department of Oral Medicine, Khyber College of Dentistry, Peshawar.
  • Sajid Hanif Department of Oral Pathology, Karachi Medical and Dental College, Karachi.
  • Faisal Pasha Department of Oral Surgery, Women Medical and Dental College, Abbottabad.
  • Muhammad Irfan khan Department of Oral & Maxillofacial Surgery, LRH Peshawar.
  • Sadaf Rauf Department of Dermatology, KTH/KMC Peshawar.
  • Zahur Qayyum Department of Oral Surgery, Women Medical and Dental College, Abbottabad.

DOI:

https://doi.org/10.33279/jkcd.v10i03.203

Keywords:

Tobacco, Nicotine, Epithelium, Oral mucosa, Morphology

Abstract

Objective:

To investigate the effect of nicotine on the morphology of an inflamed reconstituted oral mucosa in vitro when treated for 24 hours.

Materials and Methods:

This observational laboratory-based study focuses on the effects of nicotine on an in vitro reconstituted model oral mucosa. The reconstituted human epithelium model used in the study was prepared and supplied by Skin Ethic Laboratories, Nice, France. The effect of the different treatments of nicotine on tissue morphology was assessed using formalin-fixed paraffin wax sections and haematoxylin and eosin staining.

Results:

It was found that the effect of nicotine after 24 hours with working solutions (10μM and 10mM) used on inflamed oral mucosal did not significantly affect on gross morphology.

Conclusion:

The concentration of nicotine used after 24 hours did not affect tissue morphology.

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Published

2020-09-30

How to Cite

Sheikh, M. N. ., Asmat Ullah, Sajid Hanif, Faisal Pasha, Muhammad Irfan khan, Sadaf Rauf, & Zahur Qayyum. (2020). MORPHOLOGICAL EFFECT OF NICOTINE ON RECONSTITUTED MODEL OF AN INFLAMED ORAL MUCOSA AN IN VITRO STUDY. JOURNAL OF KHYBER COLLEGE OF DENTISTRY, 10(03), 16–20. https://doi.org/10.33279/jkcd.v10i03.203

Issue

Vol. 10 No. 03 (2020): Journal of Khyber College of Dentistry

Section

Original Article

License

Copyright (c) 2020 Muhammad Nauman Sheikh, Asmat Ullah, Sajid Hanif, Faisal Pasha, Muhammad Irfan khan, Sadaf Rauf, Zahur Qayyum

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