IN-VITRO EFFECT OF ERYTHROPOIETIN ON CELL LINES AND MEASUREMENT OF GROWTH FACTOR RELEASE

Authors

  • Sajid Hanif Oral Pathologist
  • Muhammad Nauman Sheikh Department of Oral Pathology, College of Dentistry Baqai Medical University, Karachi
  • Faisal Pasha Department of Oral Surgery, Women Medical and Dental College, Abbottabad
  • Asmatullah Department of Oral Medicine Khyber College of Dentistry Peshawar
  • Samara Rais Department of Oral Pathology, College of Dentistry Baqai Medical University, Karachi
  • Mahnoor Zahid Department of Oral Pathology, College of Dentistry Baqai Medical University, Karachi
  • Shahid Khan Department of Community Dentistry, Women Medical and Dental College, Abbottabad

DOI:

https://doi.org/10.33279/jkcd.v9i03.359

Keywords:

Erythropoietin, Angiogenesis, EGF, VEGF, ELISA

Abstract

Objective: The objective of this study was to investigate the effect of Erythropoietin on SCC-25, TR146, and FIBS cells lines and measurement of growth factor release, using the ELISA technique.

Materials and Methods: ELISA was performed to see if Erythropoietin (1, 10, 25 units) treatment in SCC-25, FIBS, and TR146 cells had any effect on the release of growth factor VEGF and EGF. Serum-free was used as a control.

Results: It was found that 10- and 25-unit concentration of Erythropoietin showed the maximum release of EGF in SCC-25. The effect on VEGF release was slightly different where 1 unit of Erythropoietin showed maximum VEGF release in FIBS and had no effect on the other cell types.

Conclusion: This study has confirmed that 10- and 25-unit concentration of Erythropoietin showed the maximum release of EGF in SCC-25. The effect on VEGF release was slightly different where 1 unit of Erythropoietin showed maximum VEGF release in FIBS. 

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Published

2019-09-30

How to Cite

Sajid Hanif, Muhammad Nauman Sheikh, Faisal Pasha, Asmatullah, Samara Rais, Mahnoor Zahid, & Shahid Khan. (2019). IN-VITRO EFFECT OF ERYTHROPOIETIN ON CELL LINES AND MEASUREMENT OF GROWTH FACTOR RELEASE. JOURNAL OF KHYBER COLLEGE OF DENTISTRY, 9(03), 57–62. https://doi.org/10.33279/jkcd.v9i03.359

Issue

Vol. 9 No. 03 (2019): Journal of Khyber College of Dentistry

Section

Original Article

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Copyright (c) 2019 Sajid Hanif, Muhammad Nauman Sheikh, Faisal Pasha, Asmatullah, Samara Rais, Mahnoor Zahid, Shahid Khan

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