IMMUNOHISTOCHEMICAL EXPRESSION OF PCNA, STRO-1 AND CD44 IN EXPERIMENTALLY PRODUCED PERIAPICAL PERIODONTITIS IN VIVO
DOI:
https://doi.org/10.33279/jkcd.v9i03.358Keywords:
PCNA, STRO-1, CD44 , MSCs. periapical periodontitis, proliferating dental stem cellsAbstract
Objective: To determine the immunohistochemical expression of PCNA, STRO-1 and CD44 and their role in the experimentally produced periapical periodontitis.
Materials and Methods: In this experimental study, Sprague-Dawley (SD) male rats (n=50), each weighing 200 were collected and pulp of each rat was extirpated from the mesial root of maxillary 1st molar for the development of peri-apical infection and the root canal was exposed to oral flora for 4 weeks. After 4 weeks, maxillary 1st molars mesial roots together with the surrounding alveolar bone and muscle tissue were excised and fixed in 10% neutral buffered formalin solution for 5 days and then decalcified in 10% EDTA solution for 4 weeks. All the tissue samples were then processed, and stained with hematoxylin and eosin, and were evaluated immunohistochemically using an anti-PCNA, anti- STRO-1, and anti- CD44 polyclonal antibodies.
Results: In this study, we assessed the periapical infection of the mesial root of the maxillary Ist molar, which was schematically developed in Sprague-Dawley (SD) male rats (n=50). Histological study of H&E stained sections reveals periapical periodontitis composed of chronic inflammatory cells mostly lymphocytes, along with the alveolar bone resorption around the apex of the mesial root of maxillary Ist molar. Immunohistochemistry showed positive staining for PCNA, STRO-1, and CD44. Strongly positively stained cells were found around the apex of the mesial root of the maxillary Ist molar.
Conclusion: Periapical inflammatory tissues expressed proliferative cell marker PCNA and Mesenchymal Stem Cell markers such as STRO-1, and CD44, suggesting the presence of MSCs. The PCNA, STRO-1, and CD44 positive cells were proliferated dental stem cells that were involved in the healing of the periapical periodontitis.
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Copyright (c) 2019 Sultan Zeb Khan, Khurshid Ali, Syed Umer Farooq
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