AN IN VITRO STUDY ON COMPARISON OF BINDING OF STATHERIN LIKE PEPTIDES ON TO HYDROXYAPTITE SURFACES, USING SCANNING MICRORADIOGRAPHY

Authors

  • Adil Umar Durrani Foundation University College of Dentistry, Rawalpindi.
  • Khawar Anwar Shahida Islam Medical & Dental College, Lodhran
  • Sultan Zeb Northwest School of Medicine Peshawar Pakistan
  • Almas Fasih Khattak Northwest School of Medicine Peshawar Pakistan
  • Batool Zara Foundation University College of Dentistry, Rawalpindi.
  • Wajahat Ghafoor Foundation University College of Dentistry, Rawalpindi.

DOI:

https://doi.org/10.33279/jkcd.v9i03.342

Keywords:

 Statherin, Peptides, Hydroxyapatite, Scanning microradiography, Amino acids

Abstract

Objective: The objective was to compare the rate of mineral loss of hydroxyapatite disc treated with five different sequences to that of hydroxyapatite disc treated with StN21 using SMR.

Materials and Methods: The aim of this study was to identify the functional domain of statherin required for its cariostatic function by measuring the efficacy of STATHERIN-like peptides with five different amino-acid sequences (StN21 1A, StN21 2A, StN21 3A, StN21 4A, and StN21 5A) at the N-terminal respective, in vitro using scanning microradiography. Hydroxyapatite discs of known (20%) porosity, used as an ideal system for enamel. Discs were covered with acid-resistant varnish on all surfaces except for the surface to be tested, and then placed in SMR cells and fixed on the SMR horizontal stage. Discs were demineralized by 0.1 M acetic acid buffered of pH 4.0 for about 72 hours.

Results: It was found that the effect exerted by the N–terminal of the StN21 peptide on the rate of Hap mineral dissolution was (44%) StN21 1A (53%), StN21 2A(31%), StN21 3A(49%), StN21 4A(46%) StN21 5A(48%),and finally phosphate buffer (1%).

Conclusion: Not only do the five amino acids in the N-terminal, i.e. aspartate, phosphated serine, and glutamic acid are involved in cariostatic activity, but also, there are other amino acids to play a vital role in inhibition of mineral loss. Moreover aspartate and phosphate serine are found to be least involved in inhibiting mineral loss when compared to another sequence

 

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Published

2019-09-30

How to Cite

Adil Umar Durrani, Khawar Anwar, Sultan Zeb, Almas Fasih Khattak, Batool Zara, & Wajahat Ghafoor. (2019). AN IN VITRO STUDY ON COMPARISON OF BINDING OF STATHERIN LIKE PEPTIDES ON TO HYDROXYAPTITE SURFACES, USING SCANNING MICRORADIOGRAPHY. JOURNAL OF KHYBER COLLEGE OF DENTISTRY, 9(03), 2–8. https://doi.org/10.33279/jkcd.v9i03.342

Issue

Vol. 9 No. 03 (2019): Journal of Khyber College of Dentistry

Section

Original Article

License

Copyright (c) 2019 Adil Umar Durrani, Khawar Anwar, Sultan Zeb, Almas Fasih Khattak, Batool Zara, Wajahat Ghafoor

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