DEVELOPMENT OF MULTIPLE MYELOMA CELL LINES WITH HIGH EXPRESSION OF HEPATOCYTE GROWTH FACTOR

Authors

  • Fouzia Qadir Department of Biochemistry North West School of Medicine Peshawar, KP Pakistan
  • Anders Sundan Department of Clinical and Molecular Medicine. Norwegian University of Science and Technology Trondheim Norway
  • Maria Mufti Department of Physiology, Fazaia Medical College Islamabad, Pakistan
  • Asad Jan Institute of Biotechnology and Genetic Engineering (IBGE), The university of Agriculture Peshawar, KP Pakistan

DOI:

https://doi.org/10.33279/jkcd.v10i04.188

Keywords:

Multiple myeloma, Hepatocyte growth factor (HGF), Gene, Overexpression

Abstract

Objective:

To determine the overexpress HGF gene in non HGF producing humane myeloma cell lines (INA-6).

Materials and Methods:

In this study, retroviral- expression constructs were designed and constructed. The sequence of the cloned fragments in the vector constructs was determined and the effect of construct was examined using qRT-PCR and Western blot analysis. At department of
cancer research & molecular medicine, norwegien university of science & technology, Trondheim Norway, in June 2016 to April 2018.

Results:

We established expression constructs based on HGF mRNA sequence. The HGF non producing human myeloma cell line INA-6 transduced with lentiviral HGF-overexpression transduction particles, were analyzed for their effect on the HGF gene expression. HGF overexpression was analyzed both at mRNA level and protein levels. Two myeloma cell line INA-6 HGF-1 and INA-6HGF-2 showed significant over expression of HGF mRNA and protein.

Conclusion:

In conclusion, the HGF overexpression cell lines can be used as tissue culture models to investigate the role of HGF protein in pathology of multiple myeloma.

Downloads

Published

2020-12-31

How to Cite

Fouzia Qadir, Anders Sundan, Maria Mufti, & Asad Jan. (2020). DEVELOPMENT OF MULTIPLE MYELOMA CELL LINES WITH HIGH EXPRESSION OF HEPATOCYTE GROWTH FACTOR. JOURNAL OF KHYBER COLLEGE OF DENTISTRY, 10(04), 27–33. https://doi.org/10.33279/jkcd.v10i04.188

Issue

Vol. 10 No. 04 (2020): Journal of Khyber College of Dentistry

Section

Original Article

License

Copyright (c) 2020 Fouzia Qadir, Anders Sundan, Maria Mufti, Asad Jan

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

You are free to:

  1. Share — copy and redistribute the material in any medium or format
  2. Adapt — remix, transform, and build upon the material
  3. The licensor cannot revoke these freedoms as long as you follow the license terms.

Under the following terms:

  1. Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  2. NonCommercial — You may not use the material for commercial purposes .
  3. No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.