DETECTION OF PERIODONTAL PATHOGENS IN SALIVA USING THE PCR INVADER METHOD IN PATIENTS WITH CHRONIC PERIODONTITIS AND IMPLANT THERAPY

Authors

  • Sultan Zeb khan Katsutadai Dental Clinic Chiba, Japan
  • Nobuhiro Sasaki Department of Microbiology, Tokyo Dental College, Japan
  • Farhad Ali Research & Development Cell Khyber College of Dentistry, Peshawar
  • Ritsugou Hirose Katsutadai Dental Clinic Chiba, Japan
  • Noriko Sasaki Katsutadai Dental Clinic Chiba, Japan
  • Kuniko Hirose Katsutadai Dental Clinic Chiba, Japan
  • Fumitaka Kobayashi Katsutadai Dental Clinic Chiba, Japan
  • Yuri Okamura Katsutadai Dental Clinic Chiba, Japan
  • Takanori Hayashibara Katsutadai Dental Clinic Chiba, Japan
  • Kouki Yoshikawa Katsutadai Dental Clinic Chiba, Japan
  • Kenichi Matsuzaka Department of Clinical Pathophysiology Tokyo Dental College, Japan
  • Takashi Inoue Department of Clinical Pathophysiology Tokyo Dental College, Japan

DOI:

https://doi.org/10.33279/jkcd.v9i02.352

Keywords:

Periodontal pathogens, Implant therapy, PCR invader, chronic periodontitis

Abstract

Objective:

To determine the periopathogens both prior and after the dental implantation.

Materials & Methods:

This study was conducted from March 26, 2007, to March 31, 2012, in 78 patients (21 males and 57 females) with their informed consent. As a general rule, patients who underwent tooth extraction, and received antibiotic agents due to the incision of an abscess at their initial visit were excluded. The patients were comprised of 28 chronic periodontitis patients (average age of 59.5) with a periodontal pocket exceeding seven mm;39 chronic periodontitis patients (average age of 56.0) with a periodontal pocket of 6 mm or less; and 11 patients (average age of 58.5) with healthy periodontal tissues. The index of periodontitis, including the number of existing teeth, probing depth, plaque index, and bleeding on probing (BOP) of the patients, were measured. The amount of each of the six different periodontal patogens, Aggregatibacter actinomycetemcomitans(A.a.), Porphyromonas gingivalis (P.g.), Tannerella forsythia (T.f.), Treponema denticola(T.d.), Prevotella intermedia(P.i.) and Fusobacterium ucleatum(F.n.), in the saliva of every patient was tested using the PCR-Invader method by outsourcing BML, INC., and the data were statistically processed with the Mann-Whitney U test.

Results:

In the patient group with a periodontal pocket exceeding 7 mm, there was a significant difference (P<0.05) in ratios of P.g. (0.27%) also, T.f., when compared to those of the patient group with a periodontal pocket of 6 mm or less, and those of healthy subjects (0.03%). Among patients with periodontal diseases, patients with a periodontal pocket exceeding 7 mm showed significantly higher BOP, values of the plaque index, when compared to those of patients with a periodontal pocket of 6 mm or less (P<0.05)

Conclusion:

The detection of periodontal pathogens would be useful in determining the risks of periodontitis. Also, it would be necessary to conduct examinations, before treatment of implants, for the risks of peri-implantitis.

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Published

2019-06-30

How to Cite

Sultan Zeb khan, Nobuhiro Sasaki, Farhad Ali, Ritsugou Hirose, Noriko Sasaki, Kuniko Hirose, Fumitaka Kobayashi, Yuri Okamura, Takanori Hayashibara, Kouki Yoshikawa, Kenichi Matsuzaka, & Takashi Inoue. (2019). DETECTION OF PERIODONTAL PATHOGENS IN SALIVA USING THE PCR INVADER METHOD IN PATIENTS WITH CHRONIC PERIODONTITIS AND IMPLANT THERAPY. JOURNAL OF KHYBER COLLEGE OF DENTISTRY, 9(02), 2–6. https://doi.org/10.33279/jkcd.v9i02.352

Issue

Vol. 9 No. 02 (2019): Journal of Khyber College of Dentistry

Section

Original Article

License

Copyright (c) 2019 Sultan Zeb khan, Nobuhiro Sasaki, Farhad Ali, Ritsugou Hirose, Noriko Sasaki, Kuniko Hirose, Fumitaka Kobayashi, Yuri Okamura, Takanori Hayashibara, Kouki Yoshikawa, Kenichi Matsuzaka, Takashi Inoue

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